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In an effort to validate that the particular protein species detected from the in-gel kinase assay were MAP kinases p42 and p44, immune kinase assays ended up carried out with BMM cell lysates with antibody that particularly acknowledges these two MAP kinase species. Once again, the ets-2 recombinant protein was utilized as being a substrate. These

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ended up determined by reverse transcription quantitative polymerase chain response. The outcomes are offered as histograms following normalization to ?-actin. The information are introduced as mean ± SD (The CK2 substrate specificity is invariably determined by several acidic residues Found at positions concerning -2 and +five relative to the foc

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